normal human epidermal melanocytes nhem Search Results


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Kurabo industries normal human foreskin-derived epidermal melanocyte (nhem) cells
Normal Human Foreskin Derived Epidermal Melanocyte (Nhem) Cells, supplied by Kurabo industries, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Analysis of amphiregulin (AREG) expression in senescent normal human epidermal <t>melanocytes</t> (NHEM)/BRAFm cells. ( A – D ) NHEMs were transduced with lentiviruses expressing oncogenic B-Raf V600E (BRAFm) or copepod green fluorescent protein (copGFP, NHEM/Mock) cDNA. Arrays with n = 3 sample pairs were analyzed , and the volcano blot was generated via bioinformatical analyses ( https://amp.pharm.mssm.edu/biojupies/analyze ) ( A ), AREG detection by qRT-PCR ( n = 11) ( B ), western blot analysis ( n = 10) ( C ), and ELISA ( n = 7) ( D ). The expression level of AREG was determined by Western blot analysis 7 days after infection using β-actin as a loading control. The ELISA was performed with cell culture supernatant. ( E – G ) AREG expression in vivo was detected in normal melanocytes ( n = 6) compared to nevi ( n = 6), using qRT-PCR ( E ); example of immunofluorescence staining of nevi tissue ( n = 10) and normal skin ( n = 5) (AREG: red; nucleus: blue) ( F ). AREG expression analyzed by in silico analysis of microdissected nevi ( n = 23) and primary melanoma tissues ( n = 57) generated by RNASeq analysis (Accession: GSE112509) ( G ). (*: p < 0.05; **: p < 0.01; ***: p < 0.005).
Normal Human Epidermal Melanocytes Nhem, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CellSystems Biotechnologie Vertrieb GmbH normal human epidermal melanocytes (nhem)
Analysis of amphiregulin (AREG) expression in senescent normal human epidermal <t>melanocytes</t> (NHEM)/BRAFm cells. ( A – D ) NHEMs were transduced with lentiviruses expressing oncogenic B-Raf V600E (BRAFm) or copepod green fluorescent protein (copGFP, NHEM/Mock) cDNA. Arrays with n = 3 sample pairs were analyzed , and the volcano blot was generated via bioinformatical analyses ( https://amp.pharm.mssm.edu/biojupies/analyze ) ( A ), AREG detection by qRT-PCR ( n = 11) ( B ), western blot analysis ( n = 10) ( C ), and ELISA ( n = 7) ( D ). The expression level of AREG was determined by Western blot analysis 7 days after infection using β-actin as a loading control. The ELISA was performed with cell culture supernatant. ( E – G ) AREG expression in vivo was detected in normal melanocytes ( n = 6) compared to nevi ( n = 6), using qRT-PCR ( E ); example of immunofluorescence staining of nevi tissue ( n = 10) and normal skin ( n = 5) (AREG: red; nucleus: blue) ( F ). AREG expression analyzed by in silico analysis of microdissected nevi ( n = 23) and primary melanoma tissues ( n = 57) generated by RNASeq analysis (Accession: GSE112509) ( G ). (*: p < 0.05; **: p < 0.01; ***: p < 0.005).
Normal Human Epidermal Melanocytes (Nhem), supplied by CellSystems Biotechnologie Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/normal human epidermal melanocytes (nhem)/product/CellSystems Biotechnologie Vertrieb GmbH
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BioWhittaker Molecular Applications nhem-neo (normal human epidermal melanocytes-neonatal) cell kit
Analysis of amphiregulin (AREG) expression in senescent normal human epidermal <t>melanocytes</t> (NHEM)/BRAFm cells. ( A – D ) NHEMs were transduced with lentiviruses expressing oncogenic B-Raf V600E (BRAFm) or copepod green fluorescent protein (copGFP, NHEM/Mock) cDNA. Arrays with n = 3 sample pairs were analyzed , and the volcano blot was generated via bioinformatical analyses ( https://amp.pharm.mssm.edu/biojupies/analyze ) ( A ), AREG detection by qRT-PCR ( n = 11) ( B ), western blot analysis ( n = 10) ( C ), and ELISA ( n = 7) ( D ). The expression level of AREG was determined by Western blot analysis 7 days after infection using β-actin as a loading control. The ELISA was performed with cell culture supernatant. ( E – G ) AREG expression in vivo was detected in normal melanocytes ( n = 6) compared to nevi ( n = 6), using qRT-PCR ( E ); example of immunofluorescence staining of nevi tissue ( n = 10) and normal skin ( n = 5) (AREG: red; nucleus: blue) ( F ). AREG expression analyzed by in silico analysis of microdissected nevi ( n = 23) and primary melanoma tissues ( n = 57) generated by RNASeq analysis (Accession: GSE112509) ( G ). (*: p < 0.05; **: p < 0.01; ***: p < 0.005).
Nhem Neo (Normal Human Epidermal Melanocytes Neonatal) Cell Kit, supplied by BioWhittaker Molecular Applications, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nhem-neo (normal human epidermal melanocytes-neonatal) cell kit - by Bioz Stars, 2026-06
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Lonza clonetics® normal neonatal human epidermal melanocytes (nhem)
Analysis of amphiregulin (AREG) expression in senescent normal human epidermal <t>melanocytes</t> (NHEM)/BRAFm cells. ( A – D ) NHEMs were transduced with lentiviruses expressing oncogenic B-Raf V600E (BRAFm) or copepod green fluorescent protein (copGFP, NHEM/Mock) cDNA. Arrays with n = 3 sample pairs were analyzed , and the volcano blot was generated via bioinformatical analyses ( https://amp.pharm.mssm.edu/biojupies/analyze ) ( A ), AREG detection by qRT-PCR ( n = 11) ( B ), western blot analysis ( n = 10) ( C ), and ELISA ( n = 7) ( D ). The expression level of AREG was determined by Western blot analysis 7 days after infection using β-actin as a loading control. The ELISA was performed with cell culture supernatant. ( E – G ) AREG expression in vivo was detected in normal melanocytes ( n = 6) compared to nevi ( n = 6), using qRT-PCR ( E ); example of immunofluorescence staining of nevi tissue ( n = 10) and normal skin ( n = 5) (AREG: red; nucleus: blue) ( F ). AREG expression analyzed by in silico analysis of microdissected nevi ( n = 23) and primary melanoma tissues ( n = 57) generated by RNASeq analysis (Accession: GSE112509) ( G ). (*: p < 0.05; **: p < 0.01; ***: p < 0.005).
Clonetics® Normal Neonatal Human Epidermal Melanocytes (Nhem), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Analysis of amphiregulin (AREG) expression in senescent normal human epidermal melanocytes (NHEM)/BRAFm cells. ( A – D ) NHEMs were transduced with lentiviruses expressing oncogenic B-Raf V600E (BRAFm) or copepod green fluorescent protein (copGFP, NHEM/Mock) cDNA. Arrays with n = 3 sample pairs were analyzed , and the volcano blot was generated via bioinformatical analyses ( https://amp.pharm.mssm.edu/biojupies/analyze ) ( A ), AREG detection by qRT-PCR ( n = 11) ( B ), western blot analysis ( n = 10) ( C ), and ELISA ( n = 7) ( D ). The expression level of AREG was determined by Western blot analysis 7 days after infection using β-actin as a loading control. The ELISA was performed with cell culture supernatant. ( E – G ) AREG expression in vivo was detected in normal melanocytes ( n = 6) compared to nevi ( n = 6), using qRT-PCR ( E ); example of immunofluorescence staining of nevi tissue ( n = 10) and normal skin ( n = 5) (AREG: red; nucleus: blue) ( F ). AREG expression analyzed by in silico analysis of microdissected nevi ( n = 23) and primary melanoma tissues ( n = 57) generated by RNASeq analysis (Accession: GSE112509) ( G ). (*: p < 0.05; **: p < 0.01; ***: p < 0.005).

Journal: Cells

Article Title: Amphiregulin Regulates Melanocytic Senescence

doi: 10.3390/cells10020326

Figure Lengend Snippet: Analysis of amphiregulin (AREG) expression in senescent normal human epidermal melanocytes (NHEM)/BRAFm cells. ( A – D ) NHEMs were transduced with lentiviruses expressing oncogenic B-Raf V600E (BRAFm) or copepod green fluorescent protein (copGFP, NHEM/Mock) cDNA. Arrays with n = 3 sample pairs were analyzed , and the volcano blot was generated via bioinformatical analyses ( https://amp.pharm.mssm.edu/biojupies/analyze ) ( A ), AREG detection by qRT-PCR ( n = 11) ( B ), western blot analysis ( n = 10) ( C ), and ELISA ( n = 7) ( D ). The expression level of AREG was determined by Western blot analysis 7 days after infection using β-actin as a loading control. The ELISA was performed with cell culture supernatant. ( E – G ) AREG expression in vivo was detected in normal melanocytes ( n = 6) compared to nevi ( n = 6), using qRT-PCR ( E ); example of immunofluorescence staining of nevi tissue ( n = 10) and normal skin ( n = 5) (AREG: red; nucleus: blue) ( F ). AREG expression analyzed by in silico analysis of microdissected nevi ( n = 23) and primary melanoma tissues ( n = 57) generated by RNASeq analysis (Accession: GSE112509) ( G ). (*: p < 0.05; **: p < 0.01; ***: p < 0.005).

Article Snippet: As described previously [ ], normal human epidermal melanocytes (NHEM, Lonza, Basel, Switzerland) were grown in MGM-4 BulletKit medium (Lonza).

Techniques: Expressing, Transduction, Generated, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Infection, Control, Cell Culture, In Vivo, Immunofluorescence, Staining, In Silico

Schematic overview: senescent function of AREG in melanocytes.

Journal: Cells

Article Title: Amphiregulin Regulates Melanocytic Senescence

doi: 10.3390/cells10020326

Figure Lengend Snippet: Schematic overview: senescent function of AREG in melanocytes.

Article Snippet: As described previously [ ], normal human epidermal melanocytes (NHEM, Lonza, Basel, Switzerland) were grown in MGM-4 BulletKit medium (Lonza).

Techniques: