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Image Search Results
Journal: Cells
Article Title: Amphiregulin Regulates Melanocytic Senescence
doi: 10.3390/cells10020326
Figure Lengend Snippet: Analysis of amphiregulin (AREG) expression in senescent normal human epidermal melanocytes (NHEM)/BRAFm cells. ( A – D ) NHEMs were transduced with lentiviruses expressing oncogenic B-Raf V600E (BRAFm) or copepod green fluorescent protein (copGFP, NHEM/Mock) cDNA. Arrays with n = 3 sample pairs were analyzed , and the volcano blot was generated via bioinformatical analyses ( https://amp.pharm.mssm.edu/biojupies/analyze ) ( A ), AREG detection by qRT-PCR ( n = 11) ( B ), western blot analysis ( n = 10) ( C ), and ELISA ( n = 7) ( D ). The expression level of AREG was determined by Western blot analysis 7 days after infection using β-actin as a loading control. The ELISA was performed with cell culture supernatant. ( E – G ) AREG expression in vivo was detected in normal melanocytes ( n = 6) compared to nevi ( n = 6), using qRT-PCR ( E ); example of immunofluorescence staining of nevi tissue ( n = 10) and normal skin ( n = 5) (AREG: red; nucleus: blue) ( F ). AREG expression analyzed by in silico analysis of microdissected nevi ( n = 23) and primary melanoma tissues ( n = 57) generated by RNASeq analysis (Accession: GSE112509) ( G ). (*: p < 0.05; **: p < 0.01; ***: p < 0.005).
Article Snippet: As described previously [ ],
Techniques: Expressing, Transduction, Generated, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Infection, Control, Cell Culture, In Vivo, Immunofluorescence, Staining, In Silico
Journal: Cells
Article Title: Amphiregulin Regulates Melanocytic Senescence
doi: 10.3390/cells10020326
Figure Lengend Snippet: Schematic overview: senescent function of AREG in melanocytes.
Article Snippet: As described previously [ ],
Techniques: